Western Blot Detection System

Learn more about western blotting techniques.

Western blot detection system.

It enables the detection of picogram amounts of antigen and allows for easy detection of hrp using photographic or other imaging methods. 2 0 system both accelerates immunodetection shortening time to results and enables parallel processing of blots making it easy to apply consistent conditions across experiments. Western blot is a well established and widely used technique for the detection and analysis of proteins. Western blotting is a tried and true technique in the life science lab for separating proteins based on their molecular weight.

The nano glo hibit blotting system visualizes hibit tagged proteins on blots at subpicogram levels. It is used as a general method to identify the presence of a specific single protein within a complex mixture of proteins. Protein separation and analysis. Bio rad s v3 western workflow facilitates speed and validation at each step of a western blotting experiment from running gels to quantifying proteins.

The western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein modifications such as post translational modifications at least 8 9 of all protein related publications are estimated to apply western blots. Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard ecl substrates for detection of hrp enzyme activity. We offer comprehensive protein detection solutions designed for your western blotting success. The reaction uses a detection reagent containing lgbit protein which complements the hibit tag to form the luminescent nanobit enzyme.

Quick visualization precise quantitation single probe detection or multiplexing we offer a range of reagents kits and instruments for western blot detection and analysis. Chemiluminescence is the most popular western blot detection method because of its high level of sensitivity and imaging systems geared toward use with the technique are available to further optimize speed sensitivity signal stability and ease of use.